Visium CytAssist: The Spatial Technology That Makes You The Expert
Jul 27, 2023
The Visium CytAssist is a compact, benchtop instrument that enables the transfer of transcriptomic probes from standard glass slides to Visium slides, enabling spatial profiling insights to be gained from even more samples.Compatible with hematoxylin and eosin (H&E)- or immunofluorescence (IF)-stained tissue sections, CytAssist allows pre-sectioned tissues to be used for the Visium workflow. You can further maximize your Visium experiments by screening tissue sections using standard histological techniques to find biologically significant sections and then precisely align those sections within the Visium slide Capture Area using CytAssist.
Visium CytAssist lets you begin your spatial profiling experiment by sectioning your tissue onto a standard glass slide. Just like your typical histology workflow, you can then deparaffinize, stain (H&E or IF), and image the sections. When you are ready to begin spatial profiling, the sections are de-crosslinked and hybridized with transcriptomic probes to inform the presence of a target RNA. Following probe hybridization, Visium CytAssist facilitates transfer of these transcriptomic probes from the glass slide to a Capture Area on the Visium slide. Within the instrument, two standard glass slides and a two–Capture Area Visium slide are placed so that the tissue sections on the glass slides are aligned on top of the two Visium Capture Areas. You can then proceed with the remaining steps of the Visium workflow to uncover gene expression patterns across tissue sections.